Wolverine is a mutant with a number of both natural and artificial improvements to his physiology. His primary mutant power is an accelerated healing process, typically referred to as his mutant healing factor, that regenerates damaged or destroyed tissues of his body far beyond the capabilities of an ordinary human. This power facilitated the artificial improvements he was subjected to under the Weapon X program (in later comics called the Weapon Plus program), in which his skeleton was reinforced with the virtually indestructible metal adamantium.

Wolverine's mutation also consists of animal-like adaptations of his body, including pronounced, and sharp fang-like canines and three retractable claws housed within each forearm. While originally depicted as bionic implants created by the Weapon X program, the claws are later revealed to be a natural part of his body. The claws are not made of keratin, as claws tend to be in the animal kingdom, but extremely dense bone. Wolverine's hands do not have openings for the claws to move through: they cut through his flesh every time he extrudes them, with occasional references implying that he feels a brief moment of slight pain in his hands when he unsheathes them.

Shingen had no superhuman abilities but was in peak physical condition despite his age and was one of the finest swordsmen and martial artists in all of Japan. Highly intelligent, Shingen had extensive knowledge of human anatomy, pressure points, and also great knowledge of poisons and assassination techniques. He was highly skilled in the management of criminal organizations and very well connected in the international criminal underworld, particularly in areas of drug traffic.

Silver Samurai is a mutant with the ability to generate a tachyon field. He commonly uses his power on his sword, enabling it to cut through nearly anything, except adamantium. When working with the Viper, he possesses a teleportation ring, which allows him to teleport from one location to another, granting him great mobility and stealth. As a self-styled samurai, he is a master of Kenjutsu (katana), and a martial arts master specializing in edged weapons, hand to hand combat, and tactics. He usually wears a suit of lightweight steel alloy body armor, modeled after traditional samurai armor, but made of modern protective materials. He carries a katana (long sword), shuriken (throwing stars), and other weaponry. Samurai has extensive knowledge of the operations of criminal organizations due to his shady past, which he now uses in the service of his government to combat such organizations. He is an expert in the history and customs of the samurai class (Bushido).

Viper has no superhuman abilities but her strength, speed, reflexes, agility, dexterity, coordination, balance, and endurance are of the order of an Olympic athlete. She is a great swordswoman and even greater markswoman with most long range weapons, and has extensive training in hand-to-hand combat. Viper occasionally employs poisoned weapons with snake-motifs, such as venomous darts or artificial fangs filled with poison. She utilizes experimental weaponry, including a ring that enables teleportation. A brilliant strategist and tactician with extensive combat tactical experience, Viper is highly skilled in the management of criminal organizations and very well connected in the international criminal underworld. She is also a master of stealth and espionage. Perhaps her greatest strengths are her influence, the financial resources at her disposal due to her stature in organized crime, and an uncanny luck which has allowed her to cheat death in situations where lesser people would have died. Viper may or may not have supernaturally augmented longevity.